Comparison of different methodologies to identify differentially expressed genes in two-sample cDNA microarrays
نویسندگان
چکیده
A two-sample microarray design aims at identifying genes expressed differentially in two-sample cDNA arrays. A two-sample experiment is a commonly used design to compare relative mRNA abundance between two different samples. Several statistical techniques are available for such designs. For the identification of differentially expressed genes, four methods were compared: a fold test, a t-test [3], SAM [4] and an ANOVAbased bootstrap method [2]. Mutual comparison of these methods clearly illustrates each method’s advantages and pitfalls.
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